BioTek: Microplate Instrumentation & Software Solutions
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Микропланшетный промыватель 405 Touch с сенсорным экраном

The new 405™ Touch Microplate Washer incorporates a state-of-the-art, high resolution LED backlit touch screen user interface for intuitive and flexible onboard instrument control of 96- and 384-well microplate washing. A 5.7 inch graphical color display seamlessly guides users through protocol development, instrument maintenance and daily use at the touch of a finger. Two USB flash drive ports enable convenient file transfer, storage and operation. For those who prefer computer control or require 21 CFR Part 11 compliance, the 405 Touch can be controlled with optional Liquid Handling Control™ Software. 

For over 27 years, BioTek has continued to advance the art of automated microplate washing, resulting in the industry leading ELx405™, which set the standard in full 96- and 384-well washing.  The 405 Touch, maintains all the features of the ELx405™ Microplate Washer while adding a new,  modern and powerful interface to again reshape the day-to-day routines of today’s life science laboratories.  Fast and efficient vacuum filtration, high strength biomagnetic separation, patented Dual-Action™ manifolds, optimized cell washing for loosely adherent cell monolayers and patent-pending Ultrasonic Advantage™ are all available.  New key features include automated internal 4-buffer switching and quick release manifolds for easy maintenance and modular use.

Scientists regard unexpected clogging of manifold tubes to be the largest contributor to assay failure. BioTek’s washers with their built-in ultrasonic cleaners are the only instruments of their kind available today able to automatically prime with cleaning solution, sonicate manifold tubes to remove protein or salt crystal build up and re-prime with buffer leaving the instrument clean and ready for its next wash. Cleaning these tubes is a time consuming and labor intensive process. BioTek's Ultrasonic Advantage™ incorporates the power of ultrasonic cleaning to make the 405 Touch a self-maintaining microplate washer.

For washing microsphere based assays, all 405 Touch models can be equipped with accessory modules for both vacuum filtration and biomagnetic separation. This allows for full plate washing of polystyrene and magnetic beads used in a growing number of multiplex assays and bead-based ELISAs, such as those developed on the Luminex® xMAP® technology platform. The 405 Touch’s vacuum filtration feature automates the wash steps of filter bottom plates with five different vacuum levels for optimal performance of filter pore sizes ranging from 0.45 µm to 1.2 µm. Developed with leaders in genotyping, gene expression and protein assays, the 405 Touch’s biomagnetic separation incorporates a range of immobilization options each with high-energy neodymium iron boron magnets for rapid separation of micrometer and nanometer beads with superior retention. The 405 Touch is Luminex® xMAP® approved.

The 405 Touch Washer, also known as the 405 TS Series, is available in a range of configurations for different microplate formats and wash requirements.  The 405 HT TS is designed specifically to rapidly wash 384-well microplates and includes BioTek's patented Dual-Action™ 192-tube manifold to ensure optimal performance. A proprietary tube-in-tube aspiration manifold design also provides complete 384-well evacuation even with buffers not containing surfactant.  The 405 Select TS offers the patented Dual-Action™ manifold in a 96-tube format, providing independent control of both dispense and aspirate manifolds for precise overfill washing and overflow protection. This unique design washes both 96- and 384-well plates without the need for any hardware changes. With its angled dispense tubes, full control of dispense and aspiration rates and unique X, Y, Z positioning, the 405 Select TS is ideal for the most demanding wash requirements including loosely-adherent cell lines. For those applications that use only 96-well microplates, the standard 405 TS is an ideal choice with unique features to lower background absorbance values and increase the signal-to-noise ratio of typical ELISA assays.

With its color graphical user interface and wide application range, the new 405 Touch Microplate Washer is an easy to use, total solution for 96- and 384-well plate washing in a compact and robot-compatible design. 

Applications:

  • ELISAs
  • MSD assays
  • HCS immune cytochemistry
  • FLIPR® Ca2+ flux
  • Cell-based assays
  • Magnetic and polystyrene bead processing (gene expression assays, cytokine assays)
  • ELISPOT assays

US Federal Government customers: This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note 'GSA Contract' in the Comments field).

 

xMAP® is a registered trademark of Luminex® Corporation.
FLIPR® is a registered trademark of Molecular Devices®

Hardware:

  • Accommodates 96- and 384-well microplates
  • High resolution, LED backlit touch screen UI for intuitive and flexible onboard washer control
  • 5.7 inch graphical color display seamlessly guides users through protocol creation, instrument maintenance and daily use at the touch of a finger
  • Fast Intel Atom processor running Windows CE
  • Resistive touch screen display

    - Supports all gloved environments
    - Compatible with widest range in humidity levels
    - May be operated with pointing devices
    - Low power, green technology

  • Two USB flash drive ports enable convenient file transfer, storage and operation

    - Especially helpful when instrument is shared between researchers
    - Insert memory stick, wash plates…and go
    - Each unit ships with memory stick containing sample protocols and instructional videos

  • 405 HT TS

    - Offers fast 384-well washing with patented 192-tube Dual-Action™ manifold for independent dispense and aspiration control for precise overfill washing and overflow protection

  • - Includes patent-pending Ultrasonic Advantage™ – built in ultrasonic cleaner for manifold self-maintenance
    - Equipped with superior tube-in-tube aspiration manifold design for 384-well washing with buffers not containing surfactant
    - Also calibrated for accessory 96-tube Dual-Action manifold for 96-well washing

  • 405 Select TS

    - Accommodates 96-/384-well washing with patented 96-tube Dual-Action™ manifold
    - Includes optimized cell washing with two low-flow rates and 20° angled dispense tubes ideal for washing loosely adherent cell monolayers

  • 405 TS models automate basic 96-well dispensing and aspiration
  • Automated internal 4-buffer switching
  • Internal positive displacement pump eliminates use of troublesome pressure delivery systems and allows use of any size dispense reservoir
  • Inline filtration within each dispense bottle
  • Built-in priming trough allows automatic manifold priming and rinsing during both operation and maintenance modes and eliminates need for blank plates when used in automated robotic systems
  • All washers vacuum filtration ready (accessory filtration modules sold separately)
  • All washers biomagnetic separation ready (accessory magnets sold separately)
  • Quick change manifold designs
  • Built-in fluid flow, fluid detection, and vacuum sensing provide complete protection for unattended operation
  • BioStack™ Microplate Stacker compatibility for batch processing of up to 50 ANSI/SBS standard height plates

Software:

  • Context sensitive help system complete with recommendations, diagrams and images is only a key press away when questions arise
  • Instructional videos walk users through instrument setup and basic use
  • Quick menu allows fluid paths to be easily primed, wash process started and manifold cleaned
  • Predefined protocols included for out-of-box washing

    - ELISA
    - Cell washing
    - Vacuum filtration and biomagnetic separation – Luminex® xMAP® technology workflows

  • Fully customizable protocol parameters for individualized wash requirements - gentle cell-washing to vigorous wash for high-binding assays

    - Dispense height
    - Flow rate
    - Aspiration height
    - Soak time
    - Shake time and frequency
    - Aspiration rate
    - Horizontal dispense and aspirate positions
    - Crosswise aspiration

  • AutoPrime
  • Additional software flexibility and 21 CFR Part 11 compliance with Liquid Handling Control™(LHC) Software for method development, downloading and washing plates.
  • Run protocols created onboard, downloaded from LHC™ Software or transferred on USB drive
Модель 
Кат.№
96-/384-лун.
384-лун.
96-лун
Клетки
4 промывочных раствора
УЗ очистка
Биомагнитная сепарация*
Вакуумная фильтрация*
405™ HT TS
405TSHT
 
    
405TSHTV
 
  
405™ Select TS
405TSU
   
  
405TSUV
 
 
 
405TSUS
  
 
405TSUVS
  
405™ TS
405TSR
 
 
 
 
 
405TSRV
   
 
 
405TSRS
  
 
 
405TSRVS
  
 

 

* Аксессуары к данным модулям продаются отдельно 

Конфигурация промывателя 405™ Touch 
 ОсобенностиСтроение
HTПромывка 384-лун. планшета еще быстрее
  • 192-кан (16 x 12) промывочный узел 
  • Dual-Action™
VАвтоматическое переключение м/у 4-промывочными растворами 
SUltrasonic Advantage™
  • Встроенная ультразвуковая очистка каналов
UПромывка 96-/384-лун. планшетов
  • 96-кан (8 x 12) промывочный узел 
  • Dual-Action™
Rпромывка 96-лун. планшетов
  • 96-лун (8 x 12) промывочный узел

 

 

Общая информация
Исследования ELISA
Cell-based assays (model dependent)
Magnetic bead, polystyrene bead
  • Multiplex assays
  • Bead-based ELISA

Filtration-to-waste processes
Разделение Biomagnetic separation, vacuum filtration
Типы микропланшет 96- and 384-well (model dependent)
Low profile and standard height
Solid and filter bottom
  • Filter pore sizes 0.45 µm to 1.2 µm
Встряхивание Programmable in minutes and seconds, up to 60 minutes
Intensities - slow, medium, fast or variable
Время выдержки Programmable in minutes and seconds, up to 60 minutes
Магнит High strength 96- and 384-well designs
Choice of two designs, custom for BioTek microplate washers:
Flat:
- Flat-bottom well - beads pulled to band across well bottom
- Round-bottom well - beads pulled to button at well bottom
Ring: - beads pulled to 4-zone ring at well bottom
Безопасность/Удобство/Обслуживание Waste and vacuum sensing, fluid and flow detection
Aerosol cover
Adjustment utility for plate positioning
Overflow protection
Preprogrammed maintenance routines
Автоматизация BioStack™ Microplate Stacker - up to 50 ANSI/SBS standard height plates (optional)
  • Except filter bottom plates
Встроенное программное обеспечение High resolution backlit LED touch screen UI
5.7 inch graphical color display
Intel Atom processor running Windows CE
Predefined sample wash protocols - ELISA, cell wash, biomagnetic separation and vacuum filtration
Quick Menu
Create or edit multiple custom protocols
Run protocols created onboard, downloaded from LHC™ Software or transferred on USB flash drive
Программное обеспечение Liquid Handling Control™ (LHC™), for PC wash protocol programming and execution (optional)
Рабочая температура 15° C to 30° C (59° F to 86° F)
Интерфейс For PC control and BioStack™ integration – 1 USB port, 1 9-pin RS232 port
For file transfer – 2 USB Flash Memory ports
Влажность 10% to 85%, non-condensing
Электрический 100 – 240 Volts AC 50/60 Hz, < 8.0 A
Промывание
Типы промывочного узла 96-well washing:
96-tube (8 X 12) manifold
96-/384-well washing:
Dual-Action™ 96-tube (8 X 12) manifold
384-well washing:
Dual-Action™ 192-tube (16 x 12) manifold
Скорость мойки Solid bottom plates:

96-Well:
3 asp. / disp. cycles, 300 µL/well, 96-tube manifold, final aspirate: <30 seconds
384-Well:
3 asp. / disp. cycles, 100 µL/well, 96-tube manifold, final aspirate: <80 seconds
1 asp. / disp. cycle, 400 µL/well, 384 wells, 192-tube manifold, final aspirate: <20 seconds


Filter bottom plates:
Variable, based on wash parameters
Подача жидкости One internal positive displacement pump
Вакуумная фильтрация Selectable vacuum levels (approximate finals at 30 seconds):

Lowest: -38 mmHg
Low: -113 mmHg
Medium: -200 mmHg
High: -390 mmHg
Highest: -506 mmHg

Vacuum filtration time range:
5 to 999 seconds
Диапазон объемов 25 to 3,000 µL/well
Selectable in 1 µL increments
Выбор промывочного раствора Internal automatic switching for up to 4 wash buffers (optional)
Значения расхода 1 to 11
Includes low flow, cell wash rates (model dependent)
Циклы промывания 1 to 250
Точность дозирования
  • 96-well washing - 96-tube (8 x 12) manifold:
    3% CV: 300 µL/well (deionized water with 0.1% Tween 20 and FD&C #1 blue dye), rate 6, absorbance read at 630 nm and 450 nm reference
  • 384-well washing - 192-tube (16 x 12) manifold:
    < 4% CV: 80 µL/well (deionized water with 0.1% Tween 20 and FD&C #1 blue dye), rate 7, absorbance read at 630 nm and 450 nm reference
Остаточный объем Solid bottom plates:
  • 96-well washing - 96-tube (8 x 12) manifold:
2 µL/well: 3-cycle wash, 300 µL/well (deionized water with 0.1% Tween 20), Corning 96-well flat-bottom plate, aspiration height
optimized prior to testing
  • 384-well washing - 192-tube (16 x 12) manifold:
< 2 µL/well: 3-cycle wash, 100 µL/well (deionized water with 0.1% Tween 20), Corning 96-well flat-bottom plate, aspiration height optimized prior to testing

Filter bottom plates:
  • 96-well:
Average increase weight of plate < 1.2 g after dispensing 300 µL to
0.45 µm plate, filtration 30 seconds, low vacuum, blotted
  • 384-well:
Average increase weight of plate < 4.0 g after dispensing 80 µL to 1.2
µm plate, filtration 10 seconds, low vacuum, blotted
Стерилизация Chemical
0.5% sodium hypochlorite, 70% isopropyl alcohol or 70% ethanol solution
Ультразвуковая очистка Built-in ultrasonic bath for protein and salt crystal buildup
Programmable in hours and minutes, up to 60 minutes
(model dependent)
Объем поставляемых бутылей 4 L or 10 L (optional)
Физические характеристики
Размеры Depth: 17" (43.2 cm)
Width: 14" (35.6 cm)
Height: 10" (25.4 cm)
Вес With internal buffer switching - 36 lbs (16.5 kg)
Without - 30 lbs (13.5 kg)
Регуляторный
Регуляторный For In Vitro Diagnostic use. All BioTek microplate instrumentation is CE and TUV marked.

Характеристики могут быть изменены.

Опционально

Укладчик микропланшет BioStack

Укладчик микропланшет BioStack

Укладчик для микропланшет BioStack™ добавляет дополнительную мощность следующим приборам производства BioTek Synergy™, PowerWave™, ELx405™, EL406™, MultiFlo™, MicroFlo™ Select, MicroFill™ и Precision™.


Программное обеспечение Liquid Handling Control

Программное обеспечение Liquid Handling Control

Software is a powerful yet flexible interface for use with BioTek’s liquid handlers including the 405™ Microplate Washer, the ELx405™ Select Deep Well Washer, the EL406™ Washer Dispenser and the MultiFlo™ Dispenser. New LHC Secure offers compliance to the FDA directive 21 CFR Part 11 or whenever additional security is required.


Стандартное (входит в комплект поставки)

Инструкции по применению

MANUAL Operator's manuals can be obtained via the Customer Resource Center

Опционально

BioStack Наборы для установки

7310035 BioStack Integration Kit for 405™ TS and 405™ LS

Бутыли и трубки

1173000 Dispense Bottle - with Filter, 10L
1173002 Cap for Dispense Bottle - with Filter, 10L
1173003 Cap for Dispense Bottle - with Filter, 4L
1173031 Dispense Bottle – with Filter, 4L
7100531 Cap for Waste Bottle, 4L (Also fits 10L or 20L) Доступна фотография аксессуара
7100533 Набор трубок бутыли для отработанной жидкости Доступна фотография аксессуара
7100534 Бутыль для отработанной жидкости (4л.) Доступна фотография аксессуара
7100537 Dispense Tubing Set – 4 Buffers
7100538 Набор трубок бутыли для промывочного раствора - 1 Buffer   Доступна фотография аксессуара
7100542 Бутыль для отработанной жидкости с датчиками уровня (4л.) Доступна фотография аксессуара
7100543 Waste Set with Level Sensing, 4L Bottles Доступна фотография аксессуара
7100544 Cap for Waste Bottle with Level Sensing, 4L Доступна фотография аксессуара
7100556 Бутыль для отработанной и набор трубок жидкости (20л.) Доступна фотография аксессуара
7100557 Бутыль для отработанной жидкости и набор трубок (10л.) Доступна фотография аксессуара

Системы диспенсинга/слива

1170528 Complete Dispense System, 10L Bottles
1170529 Complete Dispense System, 4L Bottles
1170530 Complete Dispense/Waste System 115V/230V, 4L Bottles
1170531 Complete High Flow Waste System 115V/230V, 10L Bottles
1170532 Complete Dispense/High Flow Waste System 115V/230V, 4L Bottles
1170533 Complete Dispense/High Flow Waste System 115V/230V, 10L Bottles
1170534 Complete Waste System 115V/230V, 10L Bottles
1170535 Complete Dispense/Waste System 115V/230V, 10L Bottles
1170536 Complete Dispense/Direct Drain Waste System 115V/230V, 4L Bottles
1170537 Complete Dispense/Direct Drain Waste System 115V/230V, 10L Bottles
7100582 Vacuum Set with Level Sense Assembly, 10L Доступна фотография аксессуара
7100746 Complete Waste System 115V/230V, 4L Bottles Доступна фотография аксессуара
7100753 Complete High Flow Waste System 115V/230V, 4L Bottles
7103024 Standard Vacuum Pump 115V/230V Доступна фотография аксессуара

Фильтры

01310 Filter for Dispense Bottle
48294 Фильтр для отработанной жидкости Доступна фотография аксессуара

Промывочные узлы и узлы диспенсинга

1170010 96-tube Dual-Action™ Manifold Kit – with 7° Dispense Manifold and Tube - in - Tube Aspiration Manifold
1172046S 96-tube Dual-Action™ Tube-in-Tube Aspiration Manifold
7102068S 96-tube Dual-Action™ 7° Dispense Manifold

Разное

01113 Silencing Muffler for Standard Vacuum Pump
1170008 96-well Vacuum Filtration Kit
1170009 96-/384-well Vacuum Filtration Kit
4030551 Счетчик вакуума/Регулятор вакуума
7100025 Набор для прочистки каналов промывочного узла  для любого промывателя ELx405™ и для ELx405™ Select c промывочным узлом, адаптированным под глубоколуночные планшеты Доступна фотография аксессуара
7102215 384-well Ring Magnet Доступна фотография аксессуара
7102216 96-well Ring Magnet Доступна фотография аксессуара
7103016 96-well Flat Magnet Доступна фотография аксессуара
7103017 384-well Flat Magnet Доступна фотография аксессуара

Квалификационные пакеты

1170522 405™ TS Qualification Package - IQ/OQ/PQ package on CD

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