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3D Cell Culture

0 uM IT1t Day 5 BF-GFP-RFP Cellular Analysis

Studies in cell biology over the last decade have shown that adherent cells aggregated in three dimensions can provide more physiologically relevant cellular responses compared to cells cultured on flat plastic- or glassware. Cell aggregation can be accomplished largely by two general methods:

  • Prevent cells from adhering to labware such that they self-aggregate into spheroids
  • Provide a three dimensional scaffold that cells can use for structure and adhere to

Each method provides challenges - regular media exchange is needed for cell viability and proliferation, and image capture poses problems that aren’t seen in typical 2D applications. BioTek’s MultiFlo™ FX with the AMX™ module is design to automate media exchange and dosing steps in many 3D assays. For spheroid image capture, the small dimensions of the sample requires quantitative microscopy to provide suitable performance in kinetic assays; in scaffolds, the sample thickness may confound results. To meet these challenges, BioTek’s Lionheart™ FX and Cytation™, along with Gen5™ 3.0 offer countless tools for imaging and analyzing 3D culture methods. Objective magnifications from 1.25x to 60x, multiple color channels, four unique modes of detection and processes such as image z-stacking, z-projections, montage mode, and image stitching provide a comprehensive solution for assays run with 3D cell culture methods.

 

HT-1080 Kinetic Spheroid Formation 24 Hour Video
Watch The Video
Biocompare Bench Tip - 3D Cell Culture
Product Tutorial:405 Touch Touchscreen Introduction

 

 

Application Notes:

Learn more about 3D Cell Culture applications - search our Application Notes and technical documentation in Resources.

 

Visual Abstracts:

BioTek’s Visual Abstracts are brief animated presentations describing the Augmented Microscopy workflow of a specific application.

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