Molecular Biology and Biochemical
Biochemical assays run the gamut from routine ELISAs to micro-volume nucleic acid and protein quantification. Endpoint and kinetic, single- and multi-wavelength assays are essential experimental tools. BioTek’s multi-mode readers have the flexibility to accommodate a very broad range of applications with superior performance.
The quantification of nucleic acids is common to many applications in basic science and clinical research. These assays use either absorbance or fluorescence to measure the concentration of DNA or RNA in the sample. Regardless of the method employed, many laboratories have adapted single cuvette protocols to a 96- and 384-well microplate-based format. These standardized formats, in conjunction with instrumentation capable of recording measurements from them, allow for the rapid quantification of large numbers of samples.
Total protein content is a measurement common to many applications in basic science and clinical research. Several different fluorescent techniques are available that eliminate many of the problems associated with the traditional absorbance-based colorimetric methods to measure total protein content.
- Receptor-Mediated Stimulation of AKTpan-kinase in NIH3T3 Cells
- Monitoring Protein Dehydrogenase Activity with Filter-based Absorbance Spectroscopy
- Analysis of the Effect of Aggregated β-Amyloid on Cellular Signaling Pathways Critical for Memory in Alzheimer’s Disease
Learn more about Biochemical Assay applications - search our Application Notes and technical documentation inResources.