Measurement of Cell Density in Culture Vessels

Reproducible cell-based assays require consistent maintenance of cell cultures and accurate evaluation of culture characteristics. Automated quantification of cell density within culture vessels enables routine passaging of cells at the optimal density and provides valuable information for conducting cell culture quality control studies. The entire area, or a representative subset of large culture vessels, including flasks and multiwell dishes, can be routinely imaged with confluence values automatically reported using the Lionheart™ FX Automated Microscope or Cytation™ Cell Imager Multi-Mode Reader.

Confluence
Automated % confluence determinations within a culture vessel

Even distribution of cells within a culture chamber promotes predictable growth kinetics and uniform characteristics within the cell population. Gen5 generated cell density reports, including mean confluence and variation, can be employed to identify and address problems with cell culturing techniques that contribute to uneven cell density across the culture vessel (see below). Additionally, this process can be used to standardize the process for identifying when to proceed with downstream applications and defining culture vessels exhibiting optimal properties.

Cell Density

 

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