Cell health and viability measurements provide essential insight into a broad range of biological processes and treatment responses. Diverse assay formats and reagents have been developed that measure specific aspects of cell viability corresponding to particular cellular response pathways and mechanisms of injury. Membrane impermeable fluorometric dyes, or apoptotic and necrotic specific markers, can be used in conjunction with label-free total cell counts to provide relative measures of cell health within a population. Activation of cellular stress pathways can be detected using expressed biosensors and detailed kinetic imaging, revealing subtle treatment-induced effects, while the comet assay and γ-H2AX immunofluorescence assay enable characterization of genotoxic-specific effects. BioTek instrumentation and Gen5 software provide automated solutions for a full spectrum of cell health and viability applications.
The ability to monitor cell stress and toxicity and its resulting effects on cellular signaling is important for both understanding disease pathology and in the drug discovery and development processes. The reversible cell stress biosensor from Montana Molecular provides a sensitive method for detecting cell stress and toxicity within a population.