BioTek Applications: Whole Organism Imaging

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Области применения
- Virology
  Research into the diverse aspects of virology are made possible by a broad range of applications and assay formats, from relatively simple methods for viral detection to those designed to elucidate complex immune responses and pathological processes. BioTek instruments provide versatile platforms for conducting viral research, with optimized imaging- and plate reader-based solutions, as well as liquid handling and automation tools for increased assay throughput and reproducibility.
- Cell Culture QC
  
  Automated imaging tools can provide valuable information for improving routine cell culturing techniques and increasing the effectiveness and reproducibility of downstream cell-based assays. Conventional methods for evaluating cell culturing techniques and assay design consist of manual inspection of a small subset of the cell population at random locations and time points.
- Cell Health and Viability
  
  Cell health and viability measurements provide essential insight into a broad range of biological processes and treatment responses. Diverse assay formats and reagents have been developed that measure specific aspects of cell viability corresponding to particular cellular response pathways and mechanisms of injury.
- Cell Metabolism
  
  Cellular metabolism comprises a number of biochemical reactions that occur in concert within the cells of living organisms. These orchestrated reactions, better described as metabolic pathways allow organisms to function and respond to environmental changes. Agilent cell metabolism assays detect discrete changes in cell bioenergetics in real time, providing a window into the critical functions that provide ATP, the energy that cells need for activation, signaling, proliferation, and biosynthesis.
- Developmental Biology
  A macro trend in drug discovery is the use of complex models of disease early in preclinical studies. An approach following this viewpoint is the use of whole organism models involving living invertebrates and vertebrates such as nematodes and zebrafish conducive to drug screening methods.
- Histology and Slide Scanning
  Histology is typically performed on fixed tissue samples using upright microscopes equipped with oculars. Cytation™ and Lionheart™ FX provide automated digital imaging of these samples using color brightfield microscopy. Low to high magnifications from 1.25x to 60x (100x with Lionheart FX) can be combined with Cytation Gen5™ image processing features such as image stitching to increase field of view.
- Immunology
  The immune system relies on diverse mechanisms working in concert to defend the host from infection and to identify and remove aberrant or damaged cells. Dysregulation of the immune system can lead to a variety of disease conditions, including autoimmune disorders, allergies, and cancer. Additionally, the immune system is a critical consideration for vaccine development and cell, tissue and organ transplants.
- Immuno-Oncology
  While harnessing the patient’s immune system to fight cancer is not a new concept, there have been significant strides in the past decade to more effectively direct and activate components of the immune system and counter the measures certain tumors adopt to shield itself from immune attack. These include immunotherapeutic drugs based on directing immune cells to tumors based on specific antigens they present, PD-1 checkpoint inhibition and the ex vivo manipulation of the patient’s own immune cells to better drive an immune response to cancer.
- Микробиология
  Since the advent of a lens system that enabled the visualization of bacteria and protists in 1671, the microscope remains a ubiquitous tool for the scientific study of live and fixed microorganisms. Investigating the structure, function, and typing of the highly diverse array of microorganisms spans heterogeneous applications including, but not limited to, the elucidation, diagnosis, and treatment of human and plant disease, food and water safety testing, vaccine development, investigation and production of industrial lipases, protein transfection to mammalian cells, environmental health and impact appraisal, horticultural and agricultural soil assessment, and exploration of alternative fuel sources.
- Molecular Biology and Biochemical
  Biochemical assays run the gamut from routine ELISAs to micro-volume nucleic acid and protein quantification. Endpoint and kinetic, single- and multi-wavelength assays are essential experimental tools. BioTek’s multi-mode readers have the flexibility to accommodate a very broad range of applications with superior performance.
- Rapid Cell Signaling
  G-protein coupled, seven-transmembrane (7-TM) receptors (GPCRs) represent large, diverse gene families found in fungi, plants and animals. The GPCR family of receptors is involved primarily with internalizing extracellular environmental signals via interaction with intracellular G-proteins. GPCR ligands and modulators range from peptide and non-peptide molecules to chemokines, nucleosides, growth factors and light.
- Stem Cell Research
  Research into the reprogramming of somatic cells into pluripotent stem cells received the Nobel Prize in 2012. The long term importance of this research resides in the use of stem cells for regenerative therapies; for the short term, however these cells represent a potential unlimited source of highly physiologically relevant cells, akin to human primary cells, that can be used as drug discovery reagents.
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  Клеточный и микробный рост
  ИФА и связанные с ним иммунологические исследования
  Количественный анализ нуклеиновых кислот
  Количественная оценка общего белка
Источники информации
- Статьи по применению
  
  BioTek’s application notes present the latest-breaking scientific content covering the vast range of applications across our entire product line. Browse applications by topic, detection and product or use the simple keyword search to find the application notes that will facilitate your research.
- Application Bulletins
  
  BioTek’s application bulletins provide brief, concise descriptions of timely applications enabled with BioTek instrumentation.
- Руководство по приложениям
  
  Many applications are straightforward and need no explanation, but best practices and tips & tricks are useful for many complex or esoteric workflows. BioTek’s application guides offer detailed information on a variety of topics.
- Публикации в журналах
  Search or browse our database to find scientific articles submitted by BioTek’s applications scientists and published in leading journals.
- Научные постеры
  BioTek’s application team has developed more than 170 scientific posters on their latest research highlighting the applications enabled with our life science instrumentation. Browse or search the topics or products of interest.
- Технические статьи
  
  Our Technical Notes illustrate product-specific capabilities and common applications for BioTek microplate instrumentation, and their implementation in common experimental design.
- Visual Abstracts
  BioTek’s Visual Abstracts are brief animated presentations describing the workflow of a single specific imaging or multi-mode detection application.
- Вебинары
  Our webinar archives are available for on-demand viewing - search to find a topic, application or product of interest. You can also register to be invited to future webinars here.
- Информационный документ
  Browse BioTek’s white papers for timely information on such important topics as Reactive Oxygen Species and 3D Cell culture.
- Ссылки
  
  Search the more than 3,000 scientific citations for BioTek’s products within our database to learn more about the applications for our life science instrumentation as they’re used around the world.
- Видео
  Here you will find a collection of videos found throughout our site. These videos can also be found on BioTek's YouTube channel.
- Предстоящие вебинары
  Through our Webinar presentations, BioTek strives to share information and experiences about timely and important microplate-based applications. Sign up today and check back for future topics that may be of interest to you.
Сервис и Поддержка
- Field Applications Scientists
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- Обслуживание на местах
  BioTek Instrument Service and Repair is available worldwide at several of our International Service Centers. Field Service is also available for many customers throughout the world.
- FAQ
  Search our Frequently Asked Questions by keyword, product or category. For additional assistance call (800) 242-4685 or submit a question via our Ask a Question form.
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О нас
- Новости
  BioTek Announces New Cytation C10 Confocal Imaging Reader
  February 2, 2021 - BioTek Instruments, a part of Agilent, is excited to unveil the Cytation C10, an advanced, automated Confocal Imaging Reader. This versatile and multi-functional automated system is ideal for research laboratories and core facilities looking to increase productivity while reaping high-quality qualitative and quantitative data to support cell-based research. Прочитать более
  
  BioTek Receives Patent for Automated Media Exchange (AMX) Module for the MultiFlo FX Multi-Mode Dispenser
  January 28, 2021 - BioTek Instruments, an Agilent Technologies, Inc., company, announces that it was granted a patent by the United States Patent and Trademark Office for the Automated Media Exchange (AMX) module for the MultiFlo FX Multi-Mode Dispenser. In cell biology workflows, the automated MultiFlo FX with AMX module enables hands-free, gentle, and highly consistent media exchanges and washing in 96- and 384-well microplate formats without disturbing sensitive cells. This promotes viability and long-term differentiation in 3-dimensional (3D) cell culture models as well as effective maintenance of loosely adherent 2-dimensional (2D) cell cultures. Прочитать более
  
  Synergy H1’s Continuously Variable Bandwidth Monochromators Optimize Assay Performance
  July 2, 2020 - BioTek Instruments announces the availability of variable bandwidth monochromators on their modular and versatile Synergy™ H1 Hybrid Multi-Mode Reader. This allows researchers to achieve even greater levels of assay sensitivity and specificity compared to fixed bandwidth systems. Прочитать более
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- События
  AACR 2021
  
  April 10 - May 21, 2021
  
  Virtual Meeting
  Week 1: April 10-15; Week 2: May 17-21
  
  BioTek instrumentation will be on display by Agilent Technologies.
  
  Online
  
  Microbiology Society Annual Conference Online 2021
  
  April 26 - 30, 2021
  
  BioTek instrumentation will be on display by BioTek U.K.
  
  Online
  
  Experimental Biology 2021 - Virtual
  
  April 27 - 30, 2021
  
  BioTek instrumentation will be on display by BioTek U.S.
  
  Online
  
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- TekTalk Newsletter
  Review past issues of TekTalk, BioTek's complimentary quarterly e-newsletter. If you are not currently subscribed, you may sign up now to begin receiving the latest life science instrumentation news.
- Карьера
  At BioTek we feel passionately and genuinely that our employees are our greatest asset. We continue to build our already impressive team having doubled our global workforce in the last five years. With our headquarters in the beautiful Green Mountains of Vermont, USA, we also have regional offices around the world.
  
  If you're interested in becoming part of our amazing team, check out our list of job openings today!
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Home Области применения Developmental Biology

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0 uM IT1t Day 5 BF-GFP-RFP Cellular Analysis

A macro trend in drug discovery is the use of complex models of disease early in preclinical studies. An approach following this viewpoint is the use of whole organism models involving living invertebrates and vertebrates such as nematodes and zebrafish conducive to drug screening methods.

Visual Abstracts (.mp4):

BioTek’s Visual Abstracts are brief animated presentations describing the Augmented Microscopy workflow of a specific application.

Measuring Apoptosis in Zebrafish
Treated with Ethanol

Related Products:

Learn more about Whole Organism Imaging applications - search our Application Notes and technical documentation in Resources.