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product design

Cytation 5 Cell Imaging Multi-Mode Reader

Многофункциональный ридер - имиджер Cytation 5
Imaging Competition Winners

Cytation™ 5 Cell Imaging Multi-Mode Reader combines automated digital widefield microscopy with conventional multi-mode microplate reading in a unique, patented design. The imaging components and multi-mode optical modules are independent and physically separate within the instrument, ensuring optimized performance without compromise! With Cytation 5, you can capture rich phenotypic cellular information and well-based quantitative data in a single experiment. Cytation 5, along with Gen5 software, automates the workflow from image capture, processing, and analysis to create publication-ready images and data. Augmented Microscopy™ is the combination of all of these features in one compact system.

Imaging Features

Multi-Mode Detection Features

System Features

8 Microplate Capacity with BioSpa8 Automated Incubator

BioSpa SystemCytation 5, integrated to BioSpa 8 Automated Incubator fully automates kinetic live cell imaging and analysis. Plate washing and reagent dispensing can be added to the system for complete walk-away automation, from sample prep through image analysis.

Benefits of the Cytation 5 & BioSpa 8 system for automated live cell imaging:

  • Full unattended workflow automation for up to 8 microplates and other labware
  • Integrated temperature and CO2/O2 control, plus humidity monitoring
  • Fluorescence, high contrast brightfield, color brightfield and phase contrast imaging
  • OnDemand mode: Remove/replace labware, independent scheduling and multi-user profiles
  • Advanced cell-level analysis including nuclear and cytoplasmic masks
  • Washing, media replacement and reagent injection



Seahorse XF Imaging and Normalization System

Cytation 5 is a key component of Agilent Technologies’ Seahorse XF Imaging and Normalization System. XFe Analyzers simultaneously measure the two major cellular energy-producing pathways, mitochondrial respiration and glycolysis, in live cells and in real time. The integrated solution with Cytation 5 combines cellular analysis and imaging for an intuitive workflow to streamline XF data interpretation.


With the ability to collect phenotypic and quantitative data in a single experiment, Cytation 5 is a powerful tool for imaging applications, and conventional multi-mode applications.

Rabbit spinal cord
Rabbit spinal cord with H&E dyes, stitched montage

SH Sy5y cells at 40x

New AutoScratch Wound Making Tool automatically creates reproducible scratch wounds in 96- and 24-well microplates for cell migration assays.

These are just a few examples of the many important applications for Cytation 5. Visit our Applications page to see more.