Promega ADCC reporter bioassay reading parameters and fold induction calculations for EC50 value

Related App Note: Automated Bioluminescent ADCC Reporter Bioassay Using Bioengineered Jurkat Cells


Gen5 version required: 2.0 or higher

Gen5 software is used to calculate the EC50 value for Promega’s ADCC reporter bioassay. Luminescence measurements indicate the level of antibody binding to the engineered effector cell. As shown in this experiment file, positive fold induction is seen with increased binding and EC50 values are derived from the dose-response curve.